3UIA
Structural Determinants of Trimerization Specificity in HIV-1 gp41 Protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-05-07 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97927 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 78.027, 76.316, 65.731 |
| Unit cell angles | 90.00, 104.86, 90.00 |
Refinement procedure
| Resolution | 63.500 - 2.000 |
| R-factor | 0.21378 |
| Rwork | 0.212 |
| R-free | 0.25227 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3u91 |
| RMSD bond length | 0.026 |
| RMSD bond angle | 1.886 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 63.500 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.060 | 0.431 |
| Number of reflections | 25339 | |
| <I/σ(I)> | 14.7 | 2.7 |
| Completeness [%] | 99.2 | 99.5 |
| Redundancy | 3.7 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.6 | 298 | 34% MPD, 0.2M ammonium dihydrogen phosphate, 0.1M Tris, pH 8.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
