3UI2
Crystal structure of the cpSRP54 tail bound to cpSRP43
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-11-21 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.933 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 112.230, 48.520, 57.850 |
| Unit cell angles | 90.00, 90.13, 90.00 |
Refinement procedure
| Resolution | 57.831 - 3.178 |
| R-factor | 0.2098 |
| Rwork | 0.207 |
| R-free | 0.26800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3deo |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.156 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.6_289)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 57.831 | 3.350 |
| High resolution limit [Å] | 3.178 | 3.178 |
| Rmerge | 0.068 | 0.434 |
| Number of reflections | 5382 | |
| <I/σ(I)> | 14.1 | 2.2 |
| Completeness [%] | 99.7 | 98.5 |
| Redundancy | 3.3 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 291 | 0.1 M BisTris, 25% (w/v) PEG 3350, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
