3UHA
Crystal Structure of Saccharopine Dehydrogenase from Saccharomyces cervisiae complexed with NAD.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E+ SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2011-01-01 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.54178 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 64.010, 104.450, 69.080 |
| Unit cell angles | 90.00, 116.60, 90.00 |
Refinement procedure
| Resolution | 32.640 - 2.300 |
| R-factor | 0.21022 |
| Rwork | 0.207 |
| R-free | 0.26995 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ID 3UGK |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.885 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.640 | 2.420 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.122 | 0.632 |
| Number of reflections | 35383 | |
| <I/σ(I)> | 5.3 | 1.5 |
| Completeness [%] | 98.1 | 96.9 |
| Redundancy | 2.2 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | 0.1M Bis-Tris Propane, 0.22M Malonate, 24% PEG 3350, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
