3UGP

Crystal structure of RNA-polymerase sigma subunit domain 2 complexed with -10 promoter element ssDNA oligo (TATAAT)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	77
Detector technology	CCD
Collection date	2009-08-09
Detector	ADSC QUANTUM 315
Spacegroup name	P 4 2 2
Unit cell lengths	86.001, 86.001, 105.862
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.220 - 2.697
R-factor	0.198
Rwork	0.196
R-free	0.23800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ku2
RMSD bond length	0.009
RMSD bond angle	1.425
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX (1.5_2)
Refinement software	PHENIX ((PHENIX.REFINE: 1.5_2))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.800
High resolution limit [Å]	2.697	2.697
Rmerge	0.105	0.520
Number of reflections	11450
<I/σ(I)>	31.63	6.77
Completeness [%]	99.8	100
Redundancy	8.8	9.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	295	5% PEG8000, 20% PEG300, 10% glycerol, 0.15% mellitic acid, 100 mM Tris, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K