3UGK
Crystal Structure of C205S mutant and Saccharopine Dehydrogenase from Saccharomyces cerevisiae.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-10-15 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 64.950, 75.230, 75.310 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.700 - 2.010 |
| R-factor | 0.20126 |
| Rwork | 0.197 |
| R-free | 0.27275 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.022 |
| RMSD bond angle | 1.741 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.700 | 2.120 |
| High resolution limit [Å] | 2.010 | 2.010 |
| Rmerge | 0.052 | 0.295 |
| Number of reflections | 24396 | |
| <I/σ(I)> | 15.8 | 3 |
| Completeness [%] | 97.1 | 81.8 |
| Redundancy | 4 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | PEG-MME 2000, Tris buffer, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
