3UGJ

Formyl Glycinamide ribonucletide amidotransferase from Salmonella Typhimurum: Role of the ATP complexation and glutaminase domain in catalytic coupling

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE F2
Synchrotron site	CHESS
Beamline	F2
Spacegroup name	P 65
Unit cell lengths	145.983, 145.983, 141.149
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.000 - 1.780
Rwork	0.211
R-free	0.24000
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	1.360
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000
High resolution limit [Å]	1.780	1.780
Number of reflections	15661
Completeness [%]	99.2	99.2
Redundancy		3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.2	298	2M Ammonium Sulphate, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K