3UER

Crystal structure of amylosucrase from Deinococcus geothermalis in complex with turanose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	CCD
Collection date	2010-07-02
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9763
Spacegroup name	C 2 2 21
Unit cell lengths	104.730, 110.420, 115.320
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	13.000 - 2.100
R-factor	0.14908
Rwork	0.146
R-free	0.20687
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.023
RMSD bond angle	1.918
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.5.0072)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	63.450	2.210
High resolution limit [Å]	2.100	2.100
Number of reflections	39319
Completeness [%]	100.0

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	285	1.5 M sodium acetate, 0.1 M sodium cacodylate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 285.0K