3UDU
Crystal structure of putative 3-isopropylmalate dehydrogenase from Campylobacter jejuni
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-03-19 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 1 |
| Unit cell lengths | 74.717, 83.170, 111.204 |
| Unit cell angles | 94.19, 90.07, 89.64 |
Refinement procedure
| Resolution | 50.000 - 1.850 |
| R-factor | 0.19951 |
| Rwork | 0.197 |
| R-free | 0.23639 |
| Structure solution method | MIR |
| Starting model (for MR) | 1cm7 |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.817 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.880 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.093 | 0.554 |
| Number of reflections | 213853 | |
| <I/σ(I)> | 18.2 | 2 |
| Completeness [%] | 99.7 | 94.7 |
| Redundancy | 2.5 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 297 | 25%PEG3350, 0.2M Na Chloride, 0.1MHEPES, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 297K |
