3UDO
Crystal structure of putative isopropylamlate dehydrogenase from Campylobacter jejuni
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-07-06 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9772 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 74.794, 74.794, 163.787 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.300 |
| R-factor | 0.17836 |
| Rwork | 0.176 |
| R-free | 0.21718 |
| Structure solution method | SAD |
| Starting model (for MR) | none |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.786 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.340 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.063 | 0.595 |
| Number of reflections | 21382 | |
| <I/σ(I)> | 40.9 | 3.7 |
| Completeness [%] | 99.7 | 99.1 |
| Redundancy | 7.8 | 7.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 297 | 25%PEG3350, 0.2M Na Chloride, 0.1MHEPES, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 297K |
