3UDB
Crystal structure of SnRK2.6
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-12-14 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.03887 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 228.094, 98.793, 113.103 |
| Unit cell angles | 90.00, 98.48, 90.00 |
Refinement procedure
| Resolution | 37.076 - 2.567 |
| R-factor | 0.2376 |
| Rwork | 0.236 |
| R-free | 0.25940 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2fh9 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.596 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.7.1_743)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.670 |
| High resolution limit [Å] | 2.567 | 2.580 |
| Number of reflections | 78271 | |
| Completeness [%] | 99.4 | 99.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 291 | 0.1M Tris, 19% PEG4000, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
