3UD9
Crystal Structure Analysis of FGF1-Disaccharide(NI23) complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13B1 |
| Synchrotron site | NSRRC |
| Beamline | BL13B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-01-07 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 113.574, 46.930, 96.708 |
| Unit cell angles | 90.00, 125.71, 90.00 |
Refinement procedure
| Resolution | 78.520 - 2.340 |
| R-factor | 0.21609 |
| Rwork | 0.212 |
| R-free | 0.25184 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1rg8 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.453 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | EPMR |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 78.520 | 2.450 |
| High resolution limit [Å] | 2.340 | 2.340 |
| Rmerge | 0.069 | 0.330 |
| Number of reflections | 16907 | |
| <I/σ(I)> | 8.6 | 2.18 |
| Completeness [%] | 96.0 | 86.83 |
| Redundancy | 3.8 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 2M (NH4)HPO4, 0.1M sodium acetate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
