3UD1
Crystal structure of ZU5A-ZU5B domains of human erythrocyte ankyrin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-F |
| Synchrotron site | APS |
| Beamline | 21-ID-F |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-11-09 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97872 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 160.123, 40.491, 178.104 |
| Unit cell angles | 90.00, 112.68, 90.00 |
Refinement procedure
| Resolution | 29.680 - 2.000 |
| R-factor | 0.173 |
| Rwork | 0.171 |
| R-free | 0.20600 |
| Structure solution method | SAD |
| Starting model (for MR) | 3f59 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.050 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | SHARP |
| Refinement software | BUSTER (2.8.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.680 | 2.090 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.093 | |
| Number of reflections | 71819 | |
| <I/σ(I)> | 7.3 | |
| Completeness [%] | 99.3 | 99.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 283.15 | 25% ethanol, 0.1 M Tris pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 283.15K |
