3UCT
Structure of Mn2+-bound N-terminal domain of calmodulin in the presence of Zn2+
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-07-20 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.758700, 1.075000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 36.288, 35.463, 58.343 |
| Unit cell angles | 90.00, 93.12, 90.00 |
Refinement procedure
| Resolution | 36.234 - 1.900 |
| R-factor | 0.2036 |
| Rwork | 0.202 |
| R-free | 0.23430 |
| Structure solution method | SAD |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.218 |
| Data reduction software | DENZO |
| Data scaling software | SCALA |
| Phasing software | AutoSol |
| Refinement software | PHENIX (1.7.2_869) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 58.257 |
| High resolution limit [Å] | 1.900 |
| Rmerge | 0.069 |
| Number of reflections | 11882 |
| <I/σ(I)> | 26.6 |
| Completeness [%] | 100.0 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 100MM TRIS-CACODYLATE, 25MM ZNCL2, 16% PEG8000, 100MM MNCL2, 20% ETHYLENE GLYCOL, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
