3UC9

Crystal Structure of Yeast Irc6p - A Novel Type of Conserved Clathrin Accessory Protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Collection date	2009-08-15
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9793
Spacegroup name	P 65
Unit cell lengths	61.638, 61.638, 95.144
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	27.270 - 1.800
R-factor	0.1977
Rwork	0.197
R-free	0.21810
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	1.104
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHELXD
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	80.000	80.000	1.860
High resolution limit [Å]	1.800	3.880	1.800
Rmerge	0.066	0.023	0.541
Number of reflections	18511
<I/σ(I)>	13.1	45.27	3.96
Completeness [%]	97.4	96.3	96.5
Redundancy	6.9	4.8	7.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	0.2 M ammonium sulfate, 0.1 MES, 30 % w/v PEG monomethyl ether 5000, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K