3UB7
Periplasmic portion of the Helicobacter pylori chemoreceptor TlpB with acetamide bound
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-11-15 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 79.587, 81.986, 94.137 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.820 - 1.400 |
| R-factor | 0.1392 |
| Rwork | 0.138 |
| R-free | 0.16230 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.484 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | EPMR |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.430 |
| High resolution limit [Å] | 1.400 | 3.450 | 1.400 |
| Rmerge | 0.044 | 0.028 | 0.517 |
| Number of reflections | 107983 | ||
| <I/σ(I)> | 13.2 | ||
| Completeness [%] | 88.7 | 81 | 89.3 |
| Redundancy | 4 | 4.2 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | 0.1 M PIPES pH 6.5, 1.9 M ammonium sulfate, vapor diffusion, hanging drop, temperature 277K |
