3UB0
Crystal structure of the nonstructural protein 7 and 8 complex of Feline Coronavirus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.2 |
| Synchrotron site | BESSY |
| Beamline | 14.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-03-08 |
| Detector | RAYONIX MX-225 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 121.454, 160.301, 102.660 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.450 - 2.600 |
| R-factor | 0.2062 |
| Rwork | 0.205 |
| R-free | 0.22740 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.290 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | SHELXD |
| Refinement software | BUSTER (2.11.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.450 | 2.700 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.058 | 0.620 |
| Number of reflections | 31155 | |
| <I/σ(I)> | 20.38 | 3.33 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 7.23 | 7.41 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.7 | 277 | 2.24M (NH4)2HPO4, 2mM TCEP, 0.1M Tris-HCl, pH 8.7, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
