3UAU

Crystal structure of the lipoprotein JlpA

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2009-07-16
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97943
Spacegroup name	P 41 21 2
Unit cell lengths	111.632, 111.632, 170.369
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.900 - 2.700
R-factor	0.24315
Rwork	0.242
R-free	0.27221
Structure solution method	MAD
RMSD bond length	0.019
RMSD bond angle	1.669
Data reduction software	HKL-3000
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.800
High resolution limit [Å]	2.700	2.700
Rmerge	0.066	0.339
Number of reflections	27364
Completeness [%]	90.5	76
Redundancy	5.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.5	288	32-36% MPD, 20-30 mM calcium chloride, 0.1 M sodium acetate, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 288K