3UAP
Crystal structure of glutathione transferase (TARGET EFI-501774) from methylococcus capsulatus str. bath
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-10-07 |
Detector | ADSC QUANTUM 315 |
Spacegroup name | P 43 3 2 |
Unit cell lengths | 129.246, 129.246, 129.246 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.080 - 2.800 |
R-factor | 0.19464 |
Rwork | 0.193 |
R-free | 0.25921 |
Structure solution method | MR |
Starting model (for MR) | 2dsa |
RMSD bond length | 0.007 |
RMSD bond angle | 1.050 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.750 |
High resolution limit [Å] | 2.700 | 2.700 |
Number of reflections | 10645 | |
<I/σ(I)> | 8.9 | 0.8 |
Completeness [%] | 99.6 | 100 |
Redundancy | 11.9 | 11.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7 | 0.2M SODIUM CHLORIDE, 0.1M TRIS-HCL, 30% PEG3000, PH 7.0, VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 294K |