3U9Z

Crystal structure between actin and a protein construct containing the first beta-thymosin domain of drosophila ciboulot (residues 2-58) with the three mutations N26D/Q27K/D28S

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	CCD
Collection date	2006-12-16
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9300
Spacegroup name	P 21 21 21
Unit cell lengths	45.795, 75.181, 117.708
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.000 - 2.090
R-factor	0.1647
Rwork	0.160
R-free	0.22660
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1sqk
RMSD bond length	0.020
RMSD bond angle	1.452
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER (1.3.2)
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	46.000		2.100
High resolution limit [Å]	2.090	12.500	2.090
Rmerge	0.092	0.027	0.475
Number of reflections	24772	143	346
<I/σ(I)>	26.84	47.13	7.04
Completeness [%]	99.9	97.3	100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	hanging drop	6.5	298	18% PEG3350, 0.05M NaAcetate pH4.7, 0.1M MgAcetate pH6.5, 0.32M Guanidine HCl, 0.8% Dioxane, hanging drop, temperature 298K