3U8Z

human merlin FERM domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	CCD
Collection date	2008-10-16
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.999990
Spacegroup name	P 41 21 2
Unit cell lengths	105.446, 105.446, 329.997
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	26.360 - 2.640
R-factor	0.2017
Rwork	0.200
R-free	0.22940
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1h4r
RMSD bond length	0.008
RMSD bond angle	0.870
Data reduction software	XDS
Data scaling software	autoPROC
Phasing software	PHASER
Refinement software	BUSTER (2.13.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	105.450	2.780
High resolution limit [Å]	2.640	2.640
Rmerge	0.063	0.377
Number of reflections	52570
<I/σ(I)>	19.9	1.9
Completeness [%]	95.2	79.7
Redundancy	7.2	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	277	protein in 50 mM Tris, pH 8.0, 300 mM sodium chloride, reservoir: 4.5% PEG4000, 0.2 M ammonium sulfate, VAPOR DIFFUSION, HANGING DROP, temperature 277K