3U8P

Cytochrome b562 integral fusion with EGFP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I02
Synchrotron site	Diamond
Beamline	I02
Temperature [K]	100
Detector technology	CCD
Collection date	2010-12-05
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97949
Spacegroup name	P 1 21 1
Unit cell lengths	64.752, 125.200, 89.255
Unit cell angles	90.00, 90.37, 90.00

Refinement procedure

Resolution	28.940 - 2.750
R-factor	0.20317
Rwork	0.201
R-free	0.24192
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	256b 2wur
RMSD bond length	0.012
RMSD bond angle	1.508
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.820
High resolution limit [Å]	2.750	2.750
Rmerge	0.034	0.324
Number of reflections	36793
<I/σ(I)>	19.9	2.4
Completeness [%]	99.3	100
Redundancy	3.7	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6.4		0.1 M MES/NAOH, PH 6.4, 200 MM magnesium acetate and 20% (W/V) PEG 8000; for cryoprotection 16% glycerol was added to the reservoir buffer, VAPOR DIFFUSION