3U8B

Functionally selective inhibition of Group IIA phospholipase A2 reveals a role for vimentin in regulating arachidonic acid metabolism

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 14-BM-C
Synchrotron site	APS
Beamline	14-BM-C
Temperature [K]	80
Detector technology	CCD
Collection date	2006-08-19
Detector	ADSC QUANTUM 315
Wavelength(s)	0.90020
Spacegroup name	P 61 2 2
Unit cell lengths	74.683, 74.683, 89.159
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	24.446 - 2.299
R-factor	0.2096
Rwork	0.207
R-free	0.25730
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.018
RMSD bond angle	1.765
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.7.1_743))

Data quality characteristics

	Overall
Low resolution limit [Å]	24.446
High resolution limit [Å]	2.299
Number of reflections	6906
Completeness [%]	99.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.4	293	0.1 M Tris HCl pH 7.4, 10 mM CaCl2, 0.5 mM Beta-octyl glucoside and 4 M NaCl, VAPOR DIFFUSION, HANGING DROP, temperature 293K