3U7B

A new crystal structure of a Fusarium oxysporum GH10 xylanase reveals the presence of an extended loop on top of the catalytic cleft

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X13
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X13
Temperature [K]	100
Detector technology	CCD
Collection date	2009-02-20
Detector	MAR CCD 165 mm
Spacegroup name	P 41 21 2
Unit cell lengths	124.220, 124.220, 284.040
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	17.910 - 1.940
R-factor	0.2142
Rwork	0.213
R-free	0.24183
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3cui
RMSD bond length	0.008
RMSD bond angle	1.069
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	BALBES
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall
Low resolution limit [Å]	17.900
High resolution limit [Å]	1.900
Number of reflections	173419

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	289	17% PEG 10000, 0.1 M ammonium acetate, 0.1 M Bis-Tris, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K