3U75
Structure of E230A-fructofuranosidase from Schwanniomyces occidentalis complexed with fructosylnystose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 120 |
| Detector technology | CCD |
| Collection date | 2010-10-25 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | P 1 |
| Unit cell lengths | 65.207, 92.663, 117.753 |
| Unit cell angles | 89.91, 82.96, 85.82 |
Refinement procedure
| Resolution | 50.000 - 2.680 |
| R-factor | 0.24483 |
| Rwork | 0.241 |
| R-free | 0.30798 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3kf3 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.820 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0088) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 116.860 |
| High resolution limit [Å] | 2.680 |
| Rmerge | 0.220 |
| Number of reflections | 74146 |
| Completeness [%] | 96.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIQUID DIFFUSION | 6 | 293 | 16% PEG 6K, 3% MPD, 0.2M MgCl2, 0.1M Mes pH 6, LIQUID DIFFUSION, temperature 293K |
