3U74
Crystal structure of stabilized human uPAR mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-06-05 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.9791 |
| Spacegroup name | H 3 |
| Unit cell lengths | 111.235, 111.235, 78.571 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 33.040 - 2.390 |
| R-factor | 0.214 |
| Rwork | 0.213 |
| R-free | 0.25200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1YWH and 2FD6 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 2.008 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.490 |
| High resolution limit [Å] | 2.390 | 5.170 | 2.400 |
| Rmerge | 0.097 | 0.061 | 0.600 |
| <I/σ(I)> | 15.7 | ||
| Completeness [%] | 99.3 | 99.4 | 93.9 |
| Redundancy | 5.2 | 5.1 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.4 | 295 | 100 mM HEPES, pH 7.5, 2% (w/v) PEG400, 2 M ammonium sulfate, vapor diffusion, sitting drop, temperature 295.0K |
