3U73

Crystal structure of stabilized human uPAR mutant in complex with ATF

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	100
Detector technology	CCD
Collection date	2008-01-01
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.9791
Spacegroup name	P 31 2 1
Unit cell lengths	130.896, 130.896, 105.315
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.760 - 3.190
R-factor	0.218
Rwork	0.216
R-free	0.25800
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.016
RMSD bond angle	2.176
Data scaling software	SCALEPACK
Refinement software	REFMAC (5.6.0117)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	99.000	99.000	3.310
High resolution limit [Å]	3.190	6.890	3.190
Rmerge	0.107	0.049	0.977
Number of reflections	16246
<I/σ(I)>	9.8
Completeness [%]	96.4	98.9	83.1
Redundancy	8	8	4.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.4	295	0.2M NaCl, 100mM HEPES, pH7.4, 1.8 M ammonium sulfate, vapor diffusion, sitting drop, temperature 295.0K