3U6B
Ef-tu (escherichia coli) in complex with nvp-ldi028
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-10-15 |
| Detector | ADSC QUANTUM 210 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 97.246, 100.376, 156.483 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.120 |
| R-factor | 0.1994 |
| Rwork | 0.196 |
| R-free | 0.25700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.250 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | CNX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.280 |
| High resolution limit [Å] | 2.120 | 2.140 |
| Rmerge | 0.098 | 0.322 |
| Number of reflections | 39701 | |
| <I/σ(I)> | 23.7 | 8.03 |
| Completeness [%] | 91.0 | 80 |
| Redundancy | 5.9 | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 0.2M AMMONIUM ACETATE, 22% PEG 3350, 0.1M BIS-TRIS PH6.5 , VAPOR DIFFUSION, SITTING DROP, temperature 293K |
