3U5S
Selenium Substituted Human Augmenter of Liver Regeneration
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 93 |
| Detector technology | IMAGE PLATE |
| Collection date | 2011-09-07 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.54178 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 50.892, 76.878, 63.464 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 21.530 - 1.500 |
| R-factor | 0.20731 |
| Rwork | 0.205 |
| R-free | 0.24353 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3mbg |
| RMSD bond length | 0.024 |
| RMSD bond angle | 2.270 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.550 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.254 | |
| Number of reflections | 15561 | |
| <I/σ(I)> | 2.43 | |
| Completeness [%] | 36.4 | |
| Redundancy | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | VAPOR DIFFUSION, HANGING DROP, temperature 298K |
