3U5P
Crystal structure of the complex of TRIM33 PHD-Bromo and H3(1-28)K9me3K14acK18acK23ac histone peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 197 |
| Detector technology | CCD |
| Collection date | 2011-05-02 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.10000 |
| Spacegroup name | P 1 |
| Unit cell lengths | 63.426, 63.608, 124.611 |
| Unit cell angles | 90.01, 90.04, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.800 |
| R-factor | 0.21317 |
| Rwork | 0.210 |
| R-free | 0.26478 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3u5m |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.557 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Number of reflections | 47438 | |
| <I/σ(I)> | 26.6 | 2.1 |
| Completeness [%] | 98.6 | 98 |
| Redundancy | 3.4 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 0.1M lithium sulfate, 0.1 mM Tris pH 8.0 and 20% PEG3350, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
