3U5M
Crystal structure of TRIM33 PHD-Bromo in the free state
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 197 |
| Detector technology | CCD |
| Collection date | 2011-02-18 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.28215 |
| Spacegroup name | P 1 |
| Unit cell lengths | 79.527, 79.781, 134.160 |
| Unit cell angles | 89.90, 89.96, 59.97 |
Refinement procedure
| Resolution | 27.360 - 3.080 |
| R-factor | 0.2106 |
| Rwork | 0.207 |
| R-free | 0.29350 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3o33 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.263 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.7.1_743)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.360 | 3.210 |
| High resolution limit [Å] | 3.080 | 3.080 |
| Number of reflections | 50478 | |
| <I/σ(I)> | 30.2 | 2.4 |
| Completeness [%] | 96.8 | 95.7 |
| Redundancy | 5.8 | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 273 | 0.2M CaCl2, 20% PEG3350, VAPOR DIFFUSION, HANGING DROP, temperature 273K |
