3U58
Crystal Structure of the Tetrahymena telomerase processivity factor Teb1 AB
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-D |
Synchrotron site | APS |
Beamline | 21-ID-D |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-01-01 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.97944 |
Spacegroup name | P 1 |
Unit cell lengths | 83.046, 83.110, 82.885 |
Unit cell angles | 108.45, 111.59, 108.42 |
Refinement procedure
Resolution | 33.580 - 2.613 |
R-factor | 0.2232 |
Rwork | 0.219 |
R-free | 0.25870 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 1.134 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.7_650) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 100.000 | 100.000 | 2.690 |
High resolution limit [Å] | 2.600 | 5.600 | 2.600 |
Rmerge | 0.103 | ||
Number of reflections | 48660 | ||
<I/σ(I)> | 21.366 | ||
Completeness [%] | 94.7 | ||
Redundancy | 3.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 9 | 277 | 10% PEG6000, pH 9.0, VAPOR DIFFUSION, SITTING DROP, temperature 277K |