3U56

Crystal structure of mutant ribosomal protein T217V TthL1 in complex with 80nt 23S RNA from Thermus thermophilus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	BRUKER AXS MICROSTAR
Temperature [K]	110
Detector technology	IMAGE PLATE
Collection date	2011-01-18
Detector	MAR scanner 345 mm plate
Spacegroup name	P 21 21 21
Unit cell lengths	73.690, 75.410, 85.880
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.300 - 2.100
R-factor	0.194
Rwork	0.191
R-free	0.23800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ad2 1mzp
RMSD bond length	0.006
RMSD bond angle	0.935
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.6.4_486))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.300	2.200
High resolution limit [Å]	2.100	2.100
Rmerge	0.099	0.408
Number of reflections	28142
<I/σ(I)>	12.57	3.38
Completeness [%]	98.5	90
Redundancy	4.6	4.01

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		5.6	295	50mM NaCl, 2.5M Ammonium sulfate, 50mM MES, 10mM Magnesium acetate , pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 295K