3U4M

Crystal structure of ribosomal protein tthl1 in complex with 80nt 23s rna from thermus thermophilus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	BRUKER AXS MICROSTAR
Temperature [K]	110
Detector technology	CCD
Collection date	2010-12-18
Detector	Bruker Platinum 135
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	62.550, 68.900, 117.560
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.675 - 2.000
R-factor	0.1807
Rwork	0.179
R-free	0.20770
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	pdb entries 1AD2 1mzp
RMSD bond length	0.007
RMSD bond angle	1.007
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.6.4_486))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.670	2.100
High resolution limit [Å]	2.000	2.000
Rmerge	0.052	0.204
Number of reflections	33346
<I/σ(I)>	19.14	6.68
Completeness [%]	94.5	89.7
Redundancy	3.99	3.75

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	295	50mM NaCl, 2.5M Ammonium sulfate, 50mM MES, 10mM Magnesium acetate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 295K