3U4L

Cryocooled bovine profilin:actin crystal structure to 2.4 A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E SUPERBRIGHT
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2011-03-12
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.54
Spacegroup name	P 21 21 21
Unit cell lengths	38.300, 71.100, 171.500
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	85.750 - 2.400
R-factor	0.24882
Rwork	0.244
R-free	0.33054
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2btf
RMSD bond length	0.014
RMSD bond angle	1.654
Data reduction software	EVAL15
Data scaling software	SADABS
Refinement software	REFMAC (5.6.0119)

Data quality characteristics

	Overall
Low resolution limit [Å]	44.550
High resolution limit [Å]	2.400
Number of reflections	121831

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.3	277	1.3M KPO4, 0.5 mM ATP, 0.2 mM CaCl2, pH 7.3, VAPOR DIFFUSION, HANGING DROP, temperature 277K