3U43
Crystal structure of the colicin E2 DNase-Im2 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-07-08 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.87260 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 121.814, 53.277, 32.785 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.810 - 1.720 |
| R-factor | 0.1638 |
| Rwork | 0.162 |
| R-free | 0.20250 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.025 |
| RMSD bond angle | 2.140 |
| Data reduction software | XDS |
| Data scaling software | SCALA (3.3.15) |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 121.814 | 48.813 | 1.810 |
| High resolution limit [Å] | 1.714 | 5.420 | 1.710 |
| Rmerge | 0.040 | 0.427 | |
| Total number of observations | 5476 | 16598 | |
| Number of reflections | 23549 | ||
| <I/σ(I)> | 14.4 | 15.5 | 1.8 |
| Completeness [%] | 99.1 | 99.9 | 93.9 |
| Redundancy | 6.9 | 6.3 | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 0.1M MMT, 27% PEG1500, pH 7.0, vapor diffusion, sitting drop, temperature 293K |
