3U39
Crystal Structure of the apo Bacillus Stearothermophilus phosphofructokinase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-04-18 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 201.909, 113.383, 76.627 |
| Unit cell angles | 90.00, 104.03, 90.00 |
Refinement procedure
| Resolution | 28.507 - 2.792 |
| R-factor | 0.202 |
| Rwork | 0.199 |
| R-free | 0.26010 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3pfk |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.806 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.900 |
| High resolution limit [Å] | 2.792 | 2.800 |
| Rmerge | 0.115 | 0.460 |
| Number of reflections | 41129 | |
| <I/σ(I)> | 14.61 | 2.8 |
| Completeness [%] | 99.2 | |
| Redundancy | 3.2 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 0.2M Ca acetate, 0.1M Na cacodylate 6.5, 18% PEG 8000, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
