3U2X
Crystal Structure of Human Glycogenin-1 (GYG1) complexed with manganese, UDP and 1'-deoxyglucose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2010-11-22 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 |
| Unit cell lengths | 46.700, 46.860, 69.700 |
| Unit cell angles | 79.57, 88.96, 77.25 |
Refinement procedure
| Resolution | 30.000 - 1.770 |
| R-factor | 0.20002 |
| Rwork | 0.198 |
| R-free | 0.24661 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3q4s |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.553 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.460 | 1.870 |
| High resolution limit [Å] | 1.770 | 1.770 |
| Rmerge | 0.075 | 0.538 |
| Number of reflections | 50332 | |
| <I/σ(I)> | 9.8 | 2.1 |
| Completeness [%] | 91.1 | 87.6 |
| Redundancy | 3.8 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293.15 | 1.4M tri-sodium citrate dihydrate, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293.15K |
