3U1C
Anti-parallel dimer of N-terminal 98-aa fragment of smooth muscle tropomyosin alpha
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 17-ID |
Synchrotron site | APS |
Beamline | 17-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2010-11-08 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9795 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 76.654, 76.654, 79.693 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 25.000 - 1.800 |
R-factor | 0.2477 |
Rwork | 0.246 |
R-free | 0.28430 |
Structure solution method | SAD |
RMSD bond length | 0.009 |
RMSD bond angle | 1.140 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | SHELXD |
Refinement software | PHENIX ((phenix.refine: 1.6.4_486)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 1.850 |
High resolution limit [Å] | 1.750 | 1.750 |
Rmerge | 0.076 | 0.436 |
Number of reflections | 23590 | |
<I/σ(I)> | 24.9 | 4.59 |
Completeness [%] | 95.9 | 94.8 |
Redundancy | 17.76 | 12.69 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4 | 293 | 0.1 M Sodium acetate, pH 4.0, 40 mM Calcium chloride, 32-35 % 2-METHYL-2,4-PENTANEDIOL, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
1 | VAPOR DIFFUSION, HANGING DROP | 4 | 293 | 0.1 M Sodium acetate, pH 4.0, 40 mM Calcium chloride, 32-35 % 2-METHYL-2,4-PENTANEDIOL, VAPOR DIFFUSION, HANGING DROP, temperature 293K |