3U12
The pleckstrin homology (PH) domain of USP37
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Detector technology | CCD |
| Collection date | 2011-09-22 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97625 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 64.083, 64.083, 218.814 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.740 - 2.080 |
| R-factor | 0.2103 |
| Rwork | 0.209 |
| R-free | 0.23200 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.030 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.8.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.120 |
| High resolution limit [Å] | 2.080 | 2.080 |
| Rmerge | 0.049 | 0.794 |
| Number of reflections | 17041 | |
| <I/σ(I)> | 52.14 | 4.6 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 20.3 | 19.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 291 | 0.1 M Sodium acetate pH 5.6, 2.46 M ammonium sulfate, 10 mM DTT , VAPOR DIFFUSION, HANGING DROP, temperature 291K |
