3TZU

Crystal structure of a glycine cleavage system H protein (GCVH) from Mycobacterium marinum

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	100
Detector technology	CCD
Collection date	2011-09-09
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97740
Spacegroup name	P 31
Unit cell lengths	50.310, 50.310, 165.320
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.272 - 2.300
R-factor	0.187
Rwork	0.184
R-free	0.25200
Structure solution method	MR
Starting model (for MR)	3ift
RMSD bond length	0.012
RMSD bond angle	1.455
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	REFMAC (5.6.0117)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.272	2.360
High resolution limit [Å]	2.300	2.300
Rmerge	0.072	0.482
Number of reflections	20740
<I/σ(I)>	16.64	2.8
Completeness [%]	99.7	99.8
Redundancy	5.151	4.265

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.5	290	Internal tracking number 219005, JCSG screen D04: 0.1 M acetate, pH 4.5, 200 mM lithium sulfate, 30% w/v PEG8000, MymaA.01046.b.A1 PS00874 16.35 mg/mL, VAPOR DIFFUSION, SITTING DROP, temperature 290.0K