3TZR

Structure of a Riboswitch-like RNA-ligand complex from the Hepatitis C Virus Internal Ribosome Entry Site

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	173
Detector technology	IMAGE PLATE
Collection date	2011-05-31
Detector	MAR scanner 345 mm plate
Wavelength(s)	1.54
Spacegroup name	P 21 21 21
Unit cell lengths	32.054, 33.582, 81.046
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	16.791 - 2.212
R-factor	0.1815
Rwork	0.179
R-free	0.23800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2nok
RMSD bond length	0.006
RMSD bond angle	1.313
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	16.800	2.280
High resolution limit [Å]	2.200	2.200
Number of reflections	4664
Completeness [%]	99.2	75.6
Redundancy	6.7	4.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	289	10 mM magnesium sulfate, 50 mM sodium cacodylate, 2.0 M ammonium sulfate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K