3TY4
Crystal structure of homoisocitrate dehydrogenase from Schizosaccharomyces pombe
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-06-25 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97856 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 52.851, 94.452, 76.076 |
| Unit cell angles | 90.00, 106.63, 90.00 |
Refinement procedure
| Resolution | 24.860 - 1.550 |
| R-factor | 0.1893 |
| Rwork | 0.188 |
| R-free | 0.21550 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ty3 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.409 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 25.000 | 25.000 | 1.610 |
| High resolution limit [Å] | 1.550 | 3.340 | 1.550 |
| Rmerge | 0.055 | 0.035 | 0.459 |
| Number of reflections | 103345 | ||
| <I/σ(I)> | 15.3 | ||
| Completeness [%] | 99.3 | 99.6 | 94.2 |
| Redundancy | 6.9 | 7.3 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | PEG 3550, Li acetate, vapor diffusion, hanging drop, temperature 293K |
