3TXN
Crystal structure of Rpn6 from Drosophila melanogaster, native data
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2010-11-03 |
| Detector | PILATUS 6M |
| Wavelength(s) | 1.00686 |
| Spacegroup name | P 61 |
| Unit cell lengths | 161.251, 161.251, 42.080 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 2.496 |
| R-factor | 0.2182 |
| Rwork | 0.216 |
| R-free | 0.26500 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.294 |
| Data reduction software | XDS |
| Data scaling software | SCALA (3.2.25) |
| Phasing software | SHELXD |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 140.028 | 52.782 | 2.630 |
| High resolution limit [Å] | 2.496 | 7.890 | 2.496 |
| Rmerge | 0.046 | 0.029 | 0.303 |
| Total number of observations | 2278 | 10599 | |
| Number of reflections | 22030 | ||
| <I/σ(I)> | 14.4 | 20.4 | 2.5 |
| Completeness [%] | 99.3 | 98 | 99 |
| Redundancy | 3.3 | 3 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.5 | 291 | 100 mM Tris HCl pH 7.5 200 mM Li2SO4, 12% PEG-3350, vapor diffusion, temperature 291K |
