3TWH
Selenium Derivatized RNA/DNA Hybrid in complex with RNase H Catalytic Domain D132N Mutant
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12C |
| Synchrotron site | NSLS |
| Beamline | X12C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-08-12 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9797, 0.9800, 0.9795 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 80.660, 37.819, 61.937 |
| Unit cell angles | 90.00, 96.60, 90.00 |
Refinement procedure
| Resolution | 40.000 - 1.790 |
| R-factor | 0.17714 |
| Rwork | 0.175 |
| R-free | 0.20782 |
| Structure solution method | MAD |
| RMSD bond length | 0.026 |
| RMSD bond angle | 2.372 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXS |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 61.530 | 1.860 |
| High resolution limit [Å] | 1.790 | 1.790 |
| Rmerge | 0.060 | 0.278 |
| Number of reflections | 17227 | |
| <I/σ(I)> | 15.3 | |
| Completeness [%] | 98.6 | 98.6 |
| Redundancy | 7.3 | 6.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 0.1 M MES, 12% PEG 20000, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
