3TUL

Crystal structure of N-terminal region of Type III Secretion Major Translocator SipB (residues 82-226)

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-BM
Synchrotron site	APS
Beamline	22-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2011-07-16
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.97243,0.97934
Spacegroup name	P 21 2 21
Unit cell lengths	159.154, 51.144, 84.646
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.943 - 2.793
R-factor	0.299
Rwork	0.298
R-free	0.31640
Structure solution method	MAD
RMSD bond length	0.009
RMSD bond angle	1.240
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	PHENIX (1.7_650)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	31.943	2.968
High resolution limit [Å]	2.793	2.793
Rmerge	0.100	0.671
Number of reflections	17478
<I/σ(I)>	18.8	2.55
Completeness [%]	98.0	80
Redundancy	12

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.1	293	0.15 M potassium bromide, 27% PEG2000 MME, pH 7.1, VAPOR DIFFUSION, HANGING DROP, temperature 293K