3TU8
Crystal Structure of the Burkholderia Lethal Factor 1 (BLF1)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-04-14 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9163 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 37.034, 45.434, 111.405 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.000 - 1.040 |
| R-factor | 0.129 |
| Rwork | 0.129 |
| R-free | 0.16000 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 0.027 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXS |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 1.100 |
| High resolution limit [Å] | 1.040 | 1.040 |
| Rmerge | 0.090 | 0.459 |
| Number of reflections | 102823 | |
| <I/σ(I)> | 18.8 | 1.7 |
| Completeness [%] | 99.0 | 87.7 |
| Redundancy | 5.2 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 290 | 26% PEG 3350, 0.2M Sodium bromide, 0.1M bis-tris propane pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
