3TTR
Crystal structure of C-lobe of bovine lactoferrin complexed with Lidocaine at 2.27 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 300 |
| Detector technology | IMAGE PLATE |
| Collection date | 2011-09-05 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 62.671, 50.421, 65.887 |
| Unit cell angles | 90.00, 107.33, 90.00 |
Refinement procedure
| Resolution | 39.340 - 2.270 |
| R-factor | 0.221 |
| Rwork | 0.221 |
| R-free | 0.24100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3o97 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.800 |
| Data reduction software | AUTOMAR |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.800 | 2.350 |
| High resolution limit [Å] | 2.270 | 2.270 |
| Number of reflections | 17966 | |
| <I/σ(I)> | 9.3 | 2 |
| Completeness [%] | 94.8 | 95.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 0.01M Znso4, 0.1M MES, 25% PEG, Monomethyl Ether 550, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
