3TTL
Crystal structure of apo-SpuE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-09-18 |
| Detector | RIGAKU |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 64.323, 48.841, 114.334 |
| Unit cell angles | 90.00, 103.24, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.300 |
| R-factor | 0.2207 |
| Rwork | 0.218 |
| R-free | 0.26997 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ttn |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.381 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.4.0077) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.510 | 2.420 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Number of reflections | 30162 | |
| Completeness [%] | 96.8 | 79.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 288 | 0.1M Tris, 0.2M MgCl2, 25% PEG 3350, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 288K |
