3TTK
Crystal structure of apo-SpuD
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-09-15 |
| Detector | RIGAKU |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 47.769, 108.983, 228.508 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.970 |
| R-factor | 0.21876 |
| Rwork | 0.215 |
| R-free | 0.28794 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ttm |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.207 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.4.0077) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 3.060 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Number of reflections | 23272 | |
| Completeness [%] | 84.9 | 80.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 288 | 25% PEG 3350, 0.1M Mes, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 288K |
