3TT9
Crystal structure of the stable degradation fragment of human plakophilin 2 isoform a (PKP2a) C752R variant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-05-19 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 0.91841 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 46.350, 63.038, 74.953 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.420 - 1.550 |
| R-factor | 0.15994 |
| Rwork | 0.158 |
| R-free | 0.19231 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1xm9 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.595 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0066) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.000 | 1.590 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Number of reflections | 32363 | |
| <I/σ(I)> | 2.87 | |
| Completeness [%] | 99.4 | 98.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293.15 | reservoir: 22% (w/v) PEG 3350, 75 M malonic acid pH 7.0, protein: 5 mg/mL in 20 mM HEPES, NaOH pH 7.5, 0.1 M NaCl, 3 mM DTT, mix 1:1, VAPOR DIFFUSION, HANGING DROP, temperature 293.15K |
